Duplicazione del DNA dalla Memoria dell'Acqua
L'acqua è il mezzo, medium e messaggio della vita, l'arcobaleno all'interno del queale rispecchia quello nel cielo.
DNA Sequence Reconstituted from Water MemorySequenza di DNA ricreata dalla sua firma elettromagnetica in acqua pura.Luc Montagnier ha scoperto che alcune sequenze di DNA batteriche e virali, disciolti nell'acqua, provocano emissione di segnali elettromagnetici ad alte diluizioni, cosa che era abbastanza grave, (vedi [1, 2] Segnali 'Omeopatia' da DNA e elettromagnetici I segnali provenienti da HIV, SiS 48). Ora, nuovi risultati dal suo laboratorio sembrano indicare che la sequenza di DNA stessa, potrebbe essere ricostituita dal segnale elettromagnetico. Cosa che ha così stupito la comunità scientifica, che un sostenitore di primo piano, è stato nientemeno che mosso ad osservare: "Luc è sia un genio, o è pazzo" Ma alcuni fisici quantistici stanno prendendo molto sul serio, e stanno collegando le scoperte di Montagnier a decenni di ricerche che dimostrano l'sensibilità di organismi ai campi elettromagnetici estremamente deboli.
A story that goes back ten years
Luc Montagnier tells the story that began 10 years ago when he discovered the strange behaviour of a small bacterium, Mycoplasm pirum, a frequent companion to human immunodeficiency virus (HIV) infection; and like the HIV, has special affinity for the human lymphocytes (white blood cells) [3]. He was trying to separate the bacterium of about 300 nm from the virus particles of about 120 nm using filters of pore size 100 nm and 20 nm, starting with pure cultures of the bacterium on lymphocytes.
The filtrate (solution that went through the filter) was sterile, and no bacterium grew in a rich culture medium that would normally support its growth. Furthermore, polymerase chain reactions (PCR) based on primers (short starting sequences) derived from adhesin, a gene of the bacterium that had been cloned and sequenced, failed to detect any DNA in the filtrate.
But, to Montagnier’s surprise, when the filtrate was incubated with lymphocytes that were not infected with Mycoplasm (according to the most stringent tests), the bacterium was regularly recovered.
So, was there some information in the filtrate responsible for directing the synthesis of the bacterium? That marked the beginning of a long series of investigations on how DNA behaves in water, which led to the discovery that the M. pirum DNA was emitting low frequency electromagnetic waves in some diluted solutions of the filtrate in water, and this property of M. pirum DNA was soon extended to other bacterial and viral DNA [1, 2].
The instrument used to detect the electromagnetic (EM) signals consists of a solenoid (a coil of wire) that detects the magnetic component of the waves produced by the DNA solution in a plastic tube as it induces an electric current in the wire. This current is amplified and analysed in a laptop computer using special software, and the resultant signals plotted out on the computer screen.
Electromagnetic signals traced to DNA sequence
In summary, ultra-low frequency (500 – 3 000 Hz) electromagnetic (EM) signals were detected in certain dilutions of the filtrate from cultures of micro-organisms (virus, bacteria) or from the plasma of humans infected with the same agents. The same results were obtained from their extracted DNA. The EM signals are not linearly correlated with the initial number of bacterial cells before filtration. In one experiment, the EM signals were similar in suspensions of E. coli cells varying from 109 down to 10. It is an “all or none” phenomenon. The EM signals are detected only in some high water dilutions of the filtrates; for example, from 10-9 to 10-18 in some preparations.
In the case of M. pirum, an isolated single gene, adhesin (previously cloned and sequenced. 3 435 basepairs) could induce the EMS, suggesting that a short DNA sequence is sufficient to induce the signals. Similarly, a short HIV DNA sequence of 104 basepairs is enough to produce the EM signals.
Some bacteria do not produce the EM signals (at least in the range detected by the instrument), as in the case of probiotic bacteria such as Lactobacillus, and also some lab strains of E. coli used as cloning vector.
These studies have been extended to viruses, but not all virus families have been investigated. Similar EM signals were detected from some retroviruses (HIV, FeLV), hepatitis viruses (HBV, HCV), and influenza A cultures. In general, EM signals are produced by 20 nm filtrates of viral suspensions or from the extracted DNA. In the case of HIV, RNA is not a source of the EM signals, but rather, the EM signals are produced by the proviral DNA present in infected cells. In bacteria, however, the EM signals are produced by 100 nm filtrates, and not by the 20 nm filtrates. This led Montagnier’s team to suggest that nanostructures of water are carriers of the information. Although highly purified water was used, the presence of trace contaminants in the nanostructures cannot be ruled out. The production of EM signals is resistant to treatment with the enzymes RNAse, DNAse, protease, or with detergent. However, it is sensitive to heat over 70 ºC and freezing (-80 ºC). This sensitivity is reduced when dealing with purified short DNA sequences. To produce the EM signals, succession (vigorous shaking) is necessary, as well as stimulation by the electromagnetic background of very low frequency, either from natural sources (the Schumann resonances, which start at 7.83 Hz) or from artificial sources, such as the mains.
DNA sequence recreated from its electromagnetic signature in pure water
In the new experiments, a fragment of HIV DNA was taken from its long terminal repeat and used for generating EM signals. This fragment was amplified by PCR to 487 bp and 104 bp. Dilutions of the DNA were made and the production of EM signals under the ambient electromagnetic background was detected.
One of the diluted solutions (say, 10-6), which gave a positive signal, was placed in a container shielded by 1 mm think mu-metal (an alloy that absorbs EM waves). Close to it, another tube containing pure water was placed. The water content of each tube had been filtered through 450 nm and 20 nm filters and diluted from 10-2 to 10-15, as for the DNA solution. A copper solenoid is placed around the tubes and they were exposed to a low intensity electric current oscillating at 7 Hz produced by an external generator. The magnetic field produced by the external generator is maintained for 18 hours at room temperature. EM signals are then recorded from each tube. At that point, the tube containing pure water also emits EM signals at the dilutions corresponding to those giving positive EMS in the original DNA tube. This result shows that the EMS carried by the nanostructures in the water originating from the DNA has been transmitted to the pure water in 18 hours. No such transfer of EM signals was achieved when the time of exposure was less than 16 to 18 hours, or when the coil is absent, or when the generator of magnetic field was turned off, or the frequency of excitation was less than 7 Hz, or when DNA was absent in the ‘donor’ tube.
Now for the most crucial test: could the EM signals transmitted to the pure water that never had DNA in it provide sufficient information to recreate the DNA sequence? To do the test, all the ingredients necessary for synthesizing the DNA by the polymerase chain reaction – nucleotides, primers, polymerase enzyme - were added to the tube with the pure water that had gained the EM signal. The amplification was done under ordinary conditions, and the DNA produced was then run through an agarose gel electrophoresis.
A DNA band of the expected size (104 bp) was found. It was 98 percent identical to the sequence of DNA from which the EM signals originated (only 2 out of 104 basepairs were different).
The experiment was highly reproducible, 12 out of 12 times; and was also repeated with another DNA sequence from the bacterium Borrelia burgdorferi, the agent of Lyme disease.
Bringing bacterium back to life from its DNA signals?
This suggests an explanation for Montagnier’s original observation made ten years ago that the bacterium could be reconstituted from a sterile filtrate incubated with human lymphocytes. The EM signals of all the bacterium’s DNA were in the sterile filtrate. The nanostructures induced by M. pirum DNA in the filtrate carried information representing different segments of its genomic DNA. Each nanostructure, when in contact with the human lymphocytes, directs the synthesis of the corresponding DNA by the DNA polymerases in the cell. There is then a certain probability that each piece of DNA recombines within the cell to reconstruct the whole DNA genome of Mycoplasm. From there, the synthesis of the rest of the bacterium – membrane lipids, ribosomes, and proteins – could take place, thanks to the host cells. One single reconstituted Mycoplasm is sufficient to infect the lymphocytes. “All the steps assumed in the regeneration from water can be analysed and open to verification.” The researchers wrote [3].
They remind us that indeed, Craig Venter’s group had claimed to have created life by first reassembling an entire Mycoplasm genome from pieces bought off the shelf (see [4] Synthetic Life? Not By a Long Shot, SiS 47). So at least that step is not impossible.
The finding also dovetails with evidence that molecules intercommunicate by electromagnetic signals, which bring them together for biochemical reactions (see [5] The Real Bioinformatics Revolution , SiS 33). However, it raises the fundamental question of how water could store and receive electromagnetic information of such precision that a DNA sequence could be reproduced without a template, which is how it is normally done.
The answer takes us on a fascinating journey through decades of research on the exquisite sensitivity of organisms to ultraweak electromagnetic fields, and the quantum electrodynamic theory of water (see [6] Quantum Coherent Water, Non-thermal EMF Effects, & Homeopathy, and other articles in the series, SiS 51).
Riferimenti1. Ho MW. ‘Homeopathic’ siganls from DNA. Science in Society 48, 36-39, 2010.
2. Ho MW. Electromagnetic signals from HIV. Science in Society 48, 40-43, 2010.
3. Montagnier L, Aissa J, Del Giudice ED, Lavallee C, Tdeschi A and Vitiello G. DNA waves and water. Journal of Physics: Conferences Series, 2011, in print arXiv:1012.5166Ms
4. Ho MW. Synthetic life? Not by a long shot. Science in Society 47, 16-17, 2010. Science in Society 33, 42-45, 2007.
5. Ho MW. The real bioinformatics revolution. Science in Society 33, 42-45, 2007.
6. Ho MW. Quantum coherent water, non-thermal EMF effects, & homeopathy. Science in Society 51 (to appear).
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Living Rainbow H2O
L'Arcobaleno Vivente H2O
Mae-Wan HoInstitute of Science in Society, UK
Published by
World Scientific Publishing Co. Pte. Ltd.
5 Toh Tuck Link, Singapore 596224
Copyright © 2012 by World Scientific Publishing Co. Pte. Ltd.
IntroduzioneContentsPreface xiii
Acknowlegements xvii
How to Read this Book xix
Chapter 1 Rainbow Dancing in the Worm 1
Love of the Rainbow Worm 1
The Quantum Jazz Dancer 3
Intercommunication is the Key 4
Water is the Means, Medium, and Message 5
Chapter 2 Weird and Wonderful Water 7
Strangely Fit for Life 7
Water Loves Bonding 9
Huge Diversity of Supramolecular Structures 10
Water, Water in Every Guise 11
Chapter 3 Cooperative Coherent Water 15
Cooperativity through Hydrogen Bonds 15
Resonant Energy Transfer through Hydrogen-bonded Networks 17
Two-state Water 18
The Chaplin Model 19
Model Fits Well with the Data 22
Water Quasicrystals and the Golden Mean 23
Two-state Model Confirmed 25
Chapter 4 Water and Colloid Crystals: The New Age of Alchemy 29
Colloid Crystal Diversity Defies Description 29
The New Age of Alchemy in Water 30
Can Like Charges Attract? 33
Colloid Quasicrystals Self-assemble in Water 34
Chapter 5 Quantum Coherent Water 39
Quantum Effects 39
What is Quantum Coherence? 40
Quantum Coherence from NMR 41
Quantum Magnetic Signatures 45
Quantum Coherence in Symphonic Structures 48
Chapter 6 QED Water I 51
Coupling Matter to Electromagnetic Field 51
QED of Condensed Matter 51
Coherent Water as a Source of Almost-free Electrons 54
Burning Water 55
Quantum Coherent Water and Life 58
Chapter 7 QED Water II: Non-thermal EMF Effects 61
Debate over Non-thermal EMF Effects 61
The “Thermal Threshold” Fallacy 62
Specificity of Non-thermal Effects 63
Ion Cyclotron Resonance 64
Ion Cyclotron Resonance for Amino Acids 65
QED Explanation Required 66
How Ion Cyclotron Resonance Could be Explained 66
Chapter 8 QED Water III: Homeopathy 71
Homeopathy and the Memory of Water 71
DNA Emits EM Signals at High Dilution 72
DNA Sequence Recreated from Its EM Signature in Pure Water 74
Bringing Bacterium to Life from Its DNA Signals? 75
DNA EM Signals from Earth’s Field NMR 76
QED and Homeopathy 77
Quantum Biology and Consciousness Arriving 79
Chapter 9 Dancing with Ions 83
Love of Water 83
Acid and Base 83
The Importance of Ions 85
To Bond or Not to Bond 86
The Jumping Bond 86
Dancing with Multiple Partners 88
Chapter 10 Dancing with Proteins 93
The Importance of Proteins 93
The Importance of Enzymes 95
Supercool Hydration Water 96
More Hydration Shells Revealed 98
Ferroelectric Hydration Water 100
Quantum Dance of Proteins 101
Chapter 11 Dancing with DNA 105
Glorious DNA 105
Wetting DNA 107
Water Electrifies DNA 108
Quantum Chemistry and DNA Conductance 109
DNA Magnetism 113
Chapter 12 Water at Solid Interfaces 115
Life at the Interface 115
Interfacial Water in the Ideal 115
Probing the Interface Directly 117
Water Forms Massive Exclusion Zones 120
Millions of Layers Thick 123
More Revelations 123
Light Charges Up Water, QED 125
Interfacial Water is Liquid Crystalline Water 127
Of Colloid Crystals and Protein Folding 127
Chapter 13 Water Electric 131
Hopping Down a Daisy Chain 131
Charging Up the Batteries of Life 132
Proton Conduction Along Biological Membranes 133
Nanotubes, Water Transport, and Proton Wires 135
Chapter 14 Water + Air = Life 139
Special Chemistry 139
Origin of Life at Air-Water Interfaces 141
Recreating Life in the Lab 142
Chapter 15 Water Meets Air 147
Walking on Water 147
Ions at the Interface 149
Ion-specific Effects 150
Chapter 16 Water Meets Membranes 157
Membranes: the Vital Interface 157
Biological Membranes 159
Wetting Membranes 161
Ca
2+ Dehydrates Phosphate Groups 166
Chapter 17 The Rainbow Ensemble 169
How Proteins and Ions Do Water’s Quantum Jazz 169
Salt Out If You Must 169
Support for the Law of Matching Water Affinities 172
Why Quantum Jazz is Possible 173
The Big Mystery Remains 176
Water’s Effortless Action through Ions 176
Water in Confined Spaces 178
Wiggins’ Theory 179
Making ATP Without Enzymes 180
Enzyme Action Depends on Two-state Water 181
Chapter 18 True Portrait of the Cell 185
What’s Wrong with Our Picture of the Cell? 185
Protoplasm vs Cell 185
From Proto-life to Cell and Protoplasm 187
The Skeleton in the Cell 190
The Cytoskeleton in all Cells 192
Metabolic Channelling in the Cytomatrix 196
Electromagnetic Signalling and Assembly of Metabolons 197
The Fractal Cell 198
Chapter 19 Water in Nanospace 203
Structured Water at £1.50 a Bottle 203
First Sighting of Structured Water 204
The Devil in Small Nanotubes 205
Defining Phases and Structures under Confinement 207
Phase Diagram of Confined Water 207
A Diversity of Liquid Crystalline Structures 208
Water in Extended Nanospace 209
Reverse Micelles for “Superactivity” 211
Water Dynamics in Reverse Micelles 214
Chapter 20 Protein and Water in Nanospace 217
Water Nanotubes, Collagen, Acupuncture, and Energy
Medicine 217
First Calculations 219
Fast Proton Exchange Between All Structured Water 221
Phase Transitions of Water Structure 222
Protein’s Secret Water Music in Nanospace 223
More Like Nanospaces in a Living Cell and More Precise
Measurements 223
A Diversity of Correlated Protein-Water Dynamics 226
Crystallographic Structures are Misleading 227
Implications for Living Cells and Recapitulation 228
Chapter 21 Fire and Water 233
Water and Redox Chemistry of Life 233
Oxygen from Water Pivotal for Life on Earth 235
Water Crisis 237
Oxygen and the Evolution of Complex Life Forms 239
Oxygen is Stable, Abundant, and Fit for Life 240
Oxygen in Action, Past and Present 240
Oxygen and the Complexity of the Metabolic Network 242
Chapter 22 Water Fuels the Dynamo of Life 245
Water and Energy Metabolism 245
Respiration 245
Photosynthesis 249
Chapter 23 Electronic Induction Animates Life 257
Sweeping Away Old and New Cobwebs 257
What’s Really Responsible for the Membrane Potential 257
The Membrane Theory 259
Potassium Bound to the Cytoplasm 260
Potassium Bound
Selectively
Inside the Cell 261
Ling’s Association-Induction Hypothesis 263
The Unit Protoplasm 266
Ling’s Hypothesis and the Liquid Crystalline Cell 267
POM and the Exclusion of Solutes 270
Support for the Liquid Crystalline Cell and Ling’s AI Hypothesis 271
Ling’s AI Hypothesis in Contemporary Cell Biology 274
Electronic Induction Animates the Cell 277
The New Cell Biology 278
Glossary 283
References 301
Author Index 321
Subject Index 325
Jacques Benveniste la memoria dell'acqua e la farmacologia DigitaleTrasferimento Farmacologico Frequenziale TFF. Il Codice Primo. Dott. Massimo CitroEdited by FabrizioOrsoBianco - 11/7/2014, 18:36
